Calcein, AM
CALCEIN-AM
CAS: 148504-34-1
Molecular Formula: C46H46N2O23
Calcein, AM - Names and Identifiers
| Name | CALCEIN-AM |
| Synonyms | CAL-AM CALCEIN-AM NSC 689290 CALCEIN, AM Calcein, AM Calcein AM solution Calcein AM solution Calcein AcetoxyMethyl Ester 4',5'-Bis(N,N-bis(carboxymethyl)aminomethyl)fluorescein acetoxymethyl ester 3',6'-Di(O-acetyl)-2',7'-bis[N,N-bis(carboxymethyl)aminomethyl]fluoresceintetraacetoxymethylester,DMSOsolution Glycine, N,N'-((3',6'-bis(acetyloxy)-3-oxospiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-diyl)bis(methylene))bis(N-(2-((acetyloxy)methoxy)-2-oxoethyl)-, bis((acetyloxy)methyl) ester N,N'-[[3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(Methylene)]bis[N-[2-[(acetyloxy)Methoxy]-2-oxoethyl]glycine 1,1'-Bis[(acetyloxy) Methyl] Ester |
| CAS | 148504-34-1 |
| EINECS | 200-664-3 |
Calcein, AM - Physico-chemical Properties
| Molecular Formula | C46H46N2O23 |
| Molar Mass | 994.86 |
| Density | 1.49±0.1 g/cm3(Predicted) |
| Melting Point | >250℃ |
| Boling Point | 982.7±65.0 °C(Predicted) |
| Flash Point | 85°C |
| Solubility | DMSO: soluble10mg/mL, clear, colorless to slightly yellow |
| Appearance | powder |
| Color | Colorless |
| Maximum wavelength(λmax) | < 300 nm |
| pKa | 2.66±0.50(Predicted) |
| Storage Condition | -20°C |
| Refractive Index | n20/D 1.479 |
| MDL | MFCD05861516 |
| Physical and Chemical Properties | Bioactive Calcein-AM are fluorescent dyes that can penetrate cells used to determine cell viability. |
| In vitro study | The calcein-AM dye used to stain the living cells is shown to have a low spontaneousleakage rate less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM has a linear relationship with measured fluorescence values. NK cells, LAKs, and CTLs are readily detectable by this microtest. Quantitation of killing and kinetic analysis is readily performed with the test system. Calcein-AM is pH independent, better retained and more photostable. In addition, the high level of intracellular retention of calcein-AM and its low-level release after incorporation exclude possible cell-monolayer labeling and allow its use in a cell-cell interaction assay. Moreover, the bright fluorescence can easily be detected and measured by a microplate fluorescence reader. Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with intact plasma membrane. From dying or damaged cells with compromised membrane integrity or from cells expressing multidrug resistance protein (MRP), unhydrolyzed substrates and their fluorescent products are rapidly extruded from cells. The calcein-AM assay has been used to assess the cell viability, cytotoxicity and tp quantitate apoptosis. |
| In vivo study | Calcein-AM is found to be suitable for in vivo studies, because it has no deleterious effects on cell function and is, indeed, a marker of cell viability. |
Calcein, AM - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. |
| UN IDs | NA 1993 / PGIII |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 10-21 |
Calcein, AM - Reference
| Reference Show more | 1. [IF = 3.31] Xiao Xiao Wang et al."2D Dendritic Gold Nanostructures Formed on Silica Nanosheets: Transferability, Clean Surface, and Their Biomedical Application." Part Part Syst Char. 2018 Oct;35(10):1800268 Note: For some products, we can only provide some information, and we do not guarantee the authority of the information provided, for customer reference and communication purposes only. use: A non-fluorecent, MEM permeable probe that can be hydroized to a fluorecent molecule storage conditions: -20 ℃ sensitivity: sensitive to light due to the poor stability of Caldin, AM, this dyeing working solution must be ready for use and used up on the day. Note: B) Calcein at a concentration of 1/10 can also be used, AM solution replaced the medium. a) if Caldin, AM is difficult to enter cells, surfactants such as Pluronic F127 can be used. (5) cells were observed with a fluorescence microscope using a filter with an excitation wavelength of 490 nm and an emission wavelength of 515 nm. (4) cells were washed twice with PBS or appropriate buffer. (3) cells were incubated at 37 °c for 15-30 minutes. (2) add 1/10 cell culture medium volume of Caldin, AM solution to the cell culture medium. B) (1) a 1 mM Calcein, AM solution was prepared in DMSO and diluted with PBS to make a 1-50 μm Calcein, AM solution. methods of use: Since the optimal staining conditions are different for different cell lines, we recommend determining the appropriate concentrations of calein, AM and PI individually. |
Calcein, AM - Introduction
Calcein, AM is a cell staining reagent that can fluorescently label living cells. After penetrating the cell membrane and entering the cell, AM is sheared by the esterase in the cell to form a Calcein, which is retained in the cell and emits strong green fluorescence. Compared with other similar reagents (such as BCECF, AM and CFDA), Calcein, AM has very low cytotoxicity. The excitation and emission wavelengths of the Calcein are 490 nm and 515 nm respectively.
Last Update：2022-10-16 17:31:04
Calcein, AM - Reference Information
| biological activity | Calcein-AM are fluorescent dyes that can penetrate cells used to determine cell viability. |
| in vitro study | the calcein-AM dye used to stain the living cells is show to have a low spontaneousleakage rate less than 15% in 4 hours at 37 c. Dilutions of targets stained by calcein-AM has a linear relationship with measured fluorescence values. NK cells, LAKs, and CTLs are readily detectable by this microtest. Quantitation of killing and kinetic analysis is readily performed with the test system. Calcein-AM is pH independent, better retained and more photostable. In addition, the high level of intracellular retention of calcein-AM and its low-level release after incorporation exclude possible cell-monolayer labeling and allow its use in a cell-cell interaction say. Moreover, the bright fluorescence can easy be detected and measured by a microplate fluorescence reader. Calcein-AM is a highly lipophilic vitaldye that rapidly, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with contact membrane. from dying or damaged cells with compromised membrane integrity or from cells expressing multidrug resistance protein (MRP), unhydrolyzed substrates and their fluorescent products are rapidly extruded from cells. The calcein-AM assayhas been used to assess the cell viability, cytotoxicity and tp quantitate apoptosis. |
| in vivo research | Calcein-AM is found to be suitable for in vivo studies, because it has no deleterious effects on cell function and is, indeed, a marker of cell viability. |
| biological application | Calcium indicators; Zinc indicators; cytotoxicity assays; apoptosis assays; viability assays; Labile iron pool assays; chemotaxis probes; Cell adhesion probes; mitochondrial probes; P-glycoprotein probes; Multi-drug resistance probes;treating atherosclerosis,cancer;ischemic disease |
Last Update：2024-04-10 22:29:15
Calcein, AM - Nature
| maximum wavelength (& lambda;max) | < 300 nm |
| biological applications | Calcium indicators; zinc indicators; cytotoxicity assays; apoptosis assays; viability assays; labile iron pool assays; chemotaxis probes; cell adhesion probes; mitochondrial probes; P-glycoprotein probes;multi-drug resistance probes;treating atherosclerosis,cancer;ischemic disease |
Last Update：2024-04-10 22:29:15
Calcein, AM - Uses and synthesis methods
in vitro studies
The calcein-AM dye used to stain the living cells is shown to have a low spontaneousleakage rate less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM has a linear relationship with measured fluorescence values. NK cells, LAKs, and CTLs are readily detectable by this microtest. Quantitation of killing and kinetic analysis is readily performed with the test system. Calcein-AM is pH independent, better retained and more photostable. In addition, the high level of intracellular retention of calcein-AM and its low-level release after incorporation exclude possible cell-monolayer labeling and allow its use in a cell-cell interaction assay. Moreover, the bright fluorescence can easily be detected and measured by a microplate fluorescence reader. Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with intact plasma membrane. From dying or damaged cells with compromised membrane integrity or from cells expressing multidrug resistance protein (MRP), unhydrolyzed substrates and their fluorescent products are rapidly extruded from cells. The calcein-AM assay has been used to assess the cell viability, cytotoxicity and tp quantitate apoptosis.
In vivo studies
Calcein-AM is found to be suitable for in vivo studies, because it has no deleterious effects on cell function and is, indeed, a marker of cell viability.
Last Update：2024-04-10 22:29:15
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